Determination of alternariol in tomato paste using solid phase extraction and high-performance liquid chromatography with fluorescence detection.

نویسندگان

  • C A Fente
  • J Jaimez
  • B I Vázquez
  • C M Franco
  • A Cepeda
چکیده

Alternaria spp. produce a wide variety of toxic metabolites with different chemical structures. Tomato products have been considered a likely source of Alternaria toxins in the human diet because Alternaria is an important spoilage mold of tomatoes. A new method for the determination of these mycotoxins in tomato paste, involving solid phase cartridges for extraction before HPLC fluorescence detection with a reversed phase column and isocratic elution, was developed. The method was demonstrated to be linear in the range 5.2-196 ppb of alternariol (AOH) in tomato paste. Good recoveries were obtained for AOH at all levels assayed (minimum 77.2%). The detection limit of the AOH toxin in real samples of tomato paste was low, 1.93 ppb. The precision of the method was demonstrated with a good repeatability (RSD = 2.98%) and reproducibility (RSD = 9.35%).

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عنوان ژورنال:
  • The Analyst

دوره 123 11  شماره 

صفحات  -

تاریخ انتشار 1998